DNA

Part:BBa_K2112011:Design

Designed by: Fanglin Zhao   Group: iGEM16_LN-Shiyan-China   (2016-10-14)


Fragment 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adding five additional amino acid behind His-tag to ensure a distance exists between His-tag and the target protein so that the protein can work better.


Source

From other plasmid.

References